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Part:BBa_K1947014:Design

Designed by: Jie Tang   Group: iGEM16_NEFU_China   (2016-10-13)


We verify the expression effect of Mms13.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal NgoMIV site found at 253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1028


Design Notes

EGFP is derived from the laboratory and used as a reporter.


Source

Magnetic bacterium Magnetospirillum magneticum AMB-1 that can produce magnet particles, also called Magnetosome, covered by a bilayer phospholipid membrane, in which Mms13 protein is anchored. EGFP is from lab data and it is used as a reporter.

References